CD79b Polyclonal antibody

Name: CD79b Polyclonal antibody
Brand: Proteintech
SKU: 21063-1-AP

CD79b Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21063-1-AP

Synonyms

Ig-beta, IGB, Ig beta, B-cell-specific glycoprotein B29, B-cell antigen receptor complex-associated protein beta chain

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of Raji using 21063-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 21063-1-AP (CD79B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human tonsillitis using 21063-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79b antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 21063-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 21063-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 21063-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 21063-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 21063-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 21063-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 21063-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 21063-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 21063-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Raji cells
Positive IHC detected in	human tonsillitis tissue, mouse spleen tissue, mouse colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 3 publications below

Product Information

21063-1-AP targets CD79b in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CD79b fusion protein Ag15086 相同性解析による交差性が予測される生物種
Full Name	CD79b molecule, immunoglobulin-associated beta
Calculated molecular weight	229 aa, 26 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC032651
Gene symbol	CD79B
Gene ID (NCBI)	974
RRID	AB_2878803
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).

Protocols

Product Specific Protocols
WB protocol for CD79b antibody 21063-1-AP	Download protocol
IHC protocol for CD79b antibody 21063-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Front Immunol CD20+CD22+ADAM28+ B Cells in Tertiary Lymphoid Structures Promote Immunotherapy Response. Authors - Zhenghao Wu View Article
mouse	IF	Cell Discov Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure Authors - Jie Yin View Article
human	IF	J Nanobiotechnology Targeted degradation of oncogenic BCR-ABL by silencing the gene of NEDD8 E3 ligase RAPSYN Authors - Yanzi Sun View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD79b Polyclonal antibody

CD79b Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of Raji using 21063-1-AP

IHC staining of human tonsillitis using 21063-1-AP

IHC staining of human tonsillitis using 21063-1-AP

IHC staining of human tonsillitis using 21063-1-AP

IHC staining of human tonsillitis using 21063-1-AP

IHC staining of human tonsillitis using 21063-1-AP

IHC staining of mouse spleen using 21063-1-AP

IHC staining of mouse spleen using 21063-1-AP

IHC staining of mouse colon using 21063-1-AP

IHC staining of mouse colon using 21063-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CD20+CD22+ADAM28+ B Cells in Tertiary Lymphoid Structures Promote Immunotherapy Response.

Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure

Targeted degradation of oncogenic BCR-ABL by silencing the gene of NEDD8 E3 ligase RAPSYN