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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD79a Polyclonal antibody (22349-1-AP)

    WB analysis using 22349-1-AP (same clone as 22349-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22349-1-AP (CD79a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD79a Polyclonal antibody (22349-1-AP)

    IHC staining of human tonsillitis using 22349-1-AP (same clone as 22349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22349-1-AP (CD79a antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD79a Polyclonal antibody (22349-1-AP)

    IHC staining of human tonsillitis using 22349-1-AP (same clone as 22349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD79a Polyclonal antibody (22349-1-AP)

    IHC staining of human lymphoma using 22349-1-AP (same clone as 22349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD79a Polyclonal antibody (22349-1-AP)

    IHC staining of human lymphoma using 22349-1-AP (same clone as 22349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22349-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD79a Polyclonal antibody (22349-1-AP)

    IF Staining of human tonsillitis using 22349-1-AP (same clone as 22349-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD79a antibody (22349-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD79a Polyclonal antibody (22349-1-AP)

    IF Staining of human tonsillitis using 22349-1-AP (same clone as 22349-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD79a antibody (22349-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22349-1-PBS in a different storage buffer formulation.

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CD79a Polyclonal antibody, PBS Only

CD79a Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 22349-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22349-1-PBS targets CD79a in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD79a fusion protein Ag17924 相同性解析による交差性が予測される生物種
Full Name CD79a molecule, immunoglobulin-associated alpha
Calculated molecular weight 226 aa, 25 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC113733
Gene Symbol CD79A
Gene ID (NCBI) 973
RRIDAB_2879084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11912
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.