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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD79a Recombinant antibody

CD79a Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241299C9

Cat no : 84236-5-RR

Synonyms

MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD79a Recombinant antibody (84236-5-RR)

    WB analysis using 84236-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84236-5-RR (CD79a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using CD79a Recombinant antibody (84236-5-RR)

    IF Staining of Ramos using 84236-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD79A antibody (84236-5-RR, Clone: 241299C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84236-5-RR

    Affinity and Kinetic Characterization of 84236-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84236-5-RR against Human CD79a were performed. The affinity constant is 3.77 nM.

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Tested Applications

Positive WB detected inDaudi cells, Raji cells
Positive IF/ICC detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84236-5-RR targets CD79a in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD79a molecule, immunoglobulin-associated alpha
Calculated molecular weight 226 aa, 25 kDa
Observed molecular weight40-45 kDa
GenBank accession numberBC113733
Gene symbol CD79A
Gene ID (NCBI) 973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

Protocols

Product Specific Protocols
WB protocol for CD79a antibody 84236-5-RRDownload protocol
IF protocol for CD79a antibody 84236-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols