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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD79b Recombinant antibody (84234-5-RR)

    WB analysis using 84234-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84234-5-RR (CD79b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD79b Recombinant antibody (84234-5-RR)

    IF Staining of Raji using 84234-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD79b antibody (84234-5-RR, Clone: 241273B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84234-5-RR

    Affinity and Kinetic Characterization of 84234-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84234-5-RR against Human CD79b were performed. The affinity constant is 3.23 nM.

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CD79b Recombinant antibody

CD79b Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84234-5-RR
Clone No.241273B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IGB, Ig beta, B-cell-specific glycoprotein B29, B-cell antigen receptor complex-associated protein beta chain, B29

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, Ramos cells, Daudi cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84234-5-RR targets CD79b in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD79b molecule, immunoglobulin-associated beta
Calculated molecular weight 229 aa, 26 kDa
Observed molecular weight32-36 kDa
GenBank accession numberBC032651
Gene Symbol CD79B
Gene ID (NCBI) 974
RRIDAB_3671787
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP40259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).

Protocols

Product Specific Protocols
WB protocol for CD79b antibody 84234-5-RRDownload protocol
IF protocol for CD79b antibody 84234-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols