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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody

CD8 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

UCHT4

Cat no : 65559-1-MR

Synonyms

CD8A, T-cell surface glycoprotein CD8 alpha chain, p32, MAL, Leu2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Ant (65559-1-MR)

    FC experiment of human PBMCs using 65559-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (65559-1-MR, Clone: UCHT4) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Ant (65559-1-MR)

    FC experiment of human PBMCs using 65559-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (65559-1-MR, Clone: UCHT4) (red) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65559-1-MR targets CD8 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Thymocytes and Sézary T cells 相同性解析による交差性が予測される生物種
Full Name CD8a molecule
Calculated molecular weight 235 aa, 26 kDa
GenBank accession numberBC025715
Gene symbol CD8A
Gene ID (NCBI) 925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
FC protocol for CD8 antibody 65559-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols