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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Rat CD8a (OX-8) Mouse IgG2a Recombinant Antibody

CD8a Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Rat

Applications

FC

Conjugate

Unconjugated

CloneNo.

OX-8

Cat no : 65577-1-MR

Synonyms

Cd8a, CD8a molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of rat splenocytes cells using Anti-Rat CD8a (OX-8) Mouse IgG2a Recombinant Antib (65577-1-MR)

    FC experiment of rat splenocytes using 65577-1-MR

    1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD8a Mouse IgG2a Recombinant Antibody (65577-1-MR, Clone: OX-8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were then stained with APC Anti-Rat CD4. Cells were not fixed.

  • Flow cytometry (FC) experiment of rat splenocytes cells using Anti-Rat CD8 (OX-8) Mouse IgG2a Recombinant Antibo (65577-1-MR)

    FC experiment of rat splenocytes using 65577-1-MR

    1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD8 Mouse IgG2a Recombinant Antibody (65577-1-MR, Clone:OX-8) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed.

  • Flow cytometry (FC) experiment of rat splenocytes cells using Anti-Rat CD8a (OX-8) Mouse IgG2a Recombinant Antib (65577-1-MR)

    FC experiment of rat splenocytes using 65577-1-MR

    1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD8a Mouse IgG2a Recombinant Antibody (65577-1-MR, Clone:OX-8) and FITC anti-Mouse IgG2a Antibody (left), or 0.25 ug Anti-Rat CD8a (65180-1-Ig, Clone: OX-8) and FITC anti-Mouse IgG1 Antibody (right). Cells were then stained with APC Anti-Rat CD4. Cells were not fixed.

Tested Applications

Positive FC detected inrat splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65577-1-MR targets CD8a in FC applications and shows reactivity with Rat samples.

Tested Reactivity Rat
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen High-molecular-weight rat thymocyte glycoproteins 相同性解析による交差性が予測される生物種
Full Name CD8a molecule
GenBank accession numberBC088126
Gene symbol Cd8a
Gene ID (NCBI) 24930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
FC protocol for CD8a antibody 65577-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols