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Validation Data Gallery

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  • Flow cytometry (FC) experiment of Daudi cells using Anti-Human CD80 (2D10.4) (65083-1-Ig)

    FC experiment of Daudi using 65083-1-Ig

    1X10^6 Daudi cells were surface stained with 0.25 ug Anti-Human CD80 (B7-1) (65083-1-Ig, Clone: 2D10.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

  • Flow cytometry (FC) experiment of Daudi cells using Anti-Human CD80 (2D10.4) (65083-1-Ig)

    FC experiment of Daudi using 65083-1-Ig

    1x10^6 Daudi cells were surface stained with 0.2 ug Anti-Human CD80 (65083-1-Ig, Clone: 2D10.4) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

  • Flow cytometry (FC) experiment of Daudi cells using Anti-Human CD80 (2D10.4) (65083-1-Ig)

    FC experiment of Daudi using 65083-1-Ig

    1x10^6 Daudi cells were surface stained with 0.2 ug Anti-Human CD80 (65083-1-Ig, Clone: 2D10.4) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

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Anti-Human CD80 (2D10.4)

CD80 Monoclonal Antibody for FC
Cat No. 65083-1-Ig
Clone No.2D10.4
Publications(1)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

B7-1, B7 1, B7, Activation B7-1 antigen, Activation B7 1 antigen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inDaudi cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

65083-1-Ig targets CD80 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A 相同性解析による交差性が予測される生物種
Full Name CD80 molecule
Calculated molecular weight 33 kDa
GenBank accession numberBC042665
Gene Symbol CD80
Gene ID (NCBI) 941
RRIDAB_2918386
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Protocols

Product Specific Protocols
FC protocol for CD80 antibody 65083-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Proc Natl Acad Sci U S A

Development of a highly sensitive platform for protein-protein interaction detection and regulation of T cell function

Authors - Hideki Hayashi
View Article