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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD80/B7-1 Monoclonal antibody, PBS Only

CD80/B7-1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E2F10

Cat no : 66406-1-PBS

Synonyms

Activation B7 1 antigen, B7, B7 1, B7-1, BB1, CD28LG, CD28LG1, CD80, CD80 molecule, CD80/B7-1, CTLA 4 counter receptor B7.1, LAB7

Validation Data Gallery

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  • Western Blot (WB) analysis of DC2.4 cells using CD80/B7-1 Monoclonal antibody (66406-1-Ig)

    WB analysis of DC2.4 using 66406-1-Ig (same clone as 66406-1-PBS)

    DC2.4 cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80/B7-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CD80/B7-1 Monoclonal antibody (66406-1-Ig)

    WB analysis of Raji using 66406-1-Ig (same clone as 66406-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using CD80/B7-1 Monoclonal antibody (66406-1-Ig)

    WB analysis of Daudi using 66406-1-Ig (same clone as 66406-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD80/B7-1 Monoclonal antibody (66406-1-Ig)

    IHC staining of human tonsillitis using 66406-1-Ig (same clone as 66406-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66406-1-Ig (CD80 / B7-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD80/B7-1 Monoclonal antibody (66406-1-Ig)

    IHC staining of human tonsillitis using 66406-1-Ig (same clone as 66406-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66406-1-Ig (CD80 / B7-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66406-1-PBS targets CD80/B7-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD80/B7-1 fusion protein Ag5615 相同性解析による交差性が予測される生物種
Full Name CD80 molecule
Calculated molecular weight 33 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC042665
Gene symbol CD80
Gene ID (NCBI) 941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)