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Validation Data Gallery

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  • Western Blot (WB) analysis of DC2.4 cells using CD80 Monoclonal antibody (66406-1-Ig)

    WB analysis of DC2.4 using 66406-1-Ig (same clone as 66406-1-PBS)

    DC2.4 cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80/B7-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CD80 Monoclonal antibody (66406-1-Ig)

    WB analysis of Raji using 66406-1-Ig (same clone as 66406-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using CD80 Monoclonal antibody (66406-1-Ig)

    WB analysis of Daudi using 66406-1-Ig (same clone as 66406-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66406-1-PBS in a different storage buffer formulation.

CD80 Monoclonal antibody, PBS Only

CD80 Monoclonal Antibody for WB, ELISA
Cat No. 66406-1-PBS
Clone No.1E2F10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, ELISA

Synonyms

1E2F10, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66406-1-PBS targets CD80 in WB, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5615

Product name: Recombinant human B7-1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 36-228 aa of BC042665

Sequence: IHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQT

相同性解析による交差性が予測される生物種
Full Name CD80 molecule
Calculated molecular weight 33 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC042665
Gene Symbol CD80
Gene ID (NCBI) 941
RRIDAB_2827408
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP33681
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)