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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD81 (5A6) Mouse IgG2a Recombinant Antibody, PBS Only

CD81 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

5A6

Cat no : 65644-1-PBS

Synonyms

TAPA1, S5.7, CD81 molecule, CD81 antigen, 5A6

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD81 (5A6) Mouse IgG2a Recombinant Anti (65644-1-MR)

    FC experiment of human peripheral blood leukocytes using 65644-1-MR (same clone as 65644-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD81 (5A6) Mouse IgG2a Recombinant Antibody (65644-1-MR, Clone: 5A6) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65644-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CD81 (5A6) Mouse IgG2a Recombinant Anti (65644-1-MR)

    FC experiment of human peripheral blood leukocytes using 65644-1-MR (same clone as 65644-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD81 (5A6) Mouse IgG2a Recombinant Antibody (65644-1-MR, Clone: 5A6) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65644-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65644-1-PBS targets CD81 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human OCI-LY8 cell line 相同性解析による交差性が予測される生物種
Full Name CD81 molecule
Calculated molecular weight 26 kDa
GenBank accession numberBC002978
Gene symbol CD81
Gene ID (NCBI) 975
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).