• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CD81 Monoclonal antibody (66866-1-Ig)

    WB analysis using 66866-1-Ig (same clone as 66866-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HCT 116 cells using CD81 Monoclonal antibody (66866-1-Ig)

    WB analysis of HCT 116 using 66866-1-Ig (same clone as 66866-1-PBS)

    WB result of CD81 antibody (66866-1-Ig; 1:3000; room temperature for 1.5 hours) with wild-type and CD81 knockout HCT 116 cells. This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD81 Monoclonal antibody (66866-1-Ig)

    WB analysis using 66866-1-Ig (same clone as 66866-1-PBS)

    HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CD81 Monoclonal antibody (66866-1-Ig)

    WB analysis of Jurkat using 66866-1-Ig (same clone as 66866-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using CD81 Monoclonal antibody (66866-1-Ig)

    WB analysis of THP-1 using 66866-1-Ig (same clone as 66866-1-PBS)

    LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD81 Monoclonal antibody (66866-1-Ig)

    IHC staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66866-1-Ig (CD81 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD81 Monoclonal antibody (66866-1-Ig)

    IHC staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66866-1-Ig (CD81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD81 Monoclonal antibody (66866-1-Ig)

    IF Staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD81 antibody (66866-1-Ig, Clone: 1G2C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD81 Monoclonal antibody (66866-1-Ig)

    IF Staining of human tonsillitis using 66866-1-Ig (same clone as 66866-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD81 antibody (66866-1-Ig, Clone: 1G2C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66866-1-PBS in a different storage buffer formulation.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

CD81 Monoclonal antibody, PBS Only

CD81 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66866-1-PBS
Clone No.1G2C6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

TAPA1, S5.7, CD81 molecule, CD81 antigen, 26 kDa cell surface protein TAPA-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66866-1-PBS targets CD81 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD81 fusion protein Ag27298 相同性解析による交差性が予測される生物種
Full Name CD81 molecule
Calculated molecular weight 26 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC002978
Gene Symbol CD81
Gene ID (NCBI) 975
ENSEMBL Gene IDENSG00000110651
RRIDAB_2882203
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60033
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).