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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD81 Recombinant antibody

CD81 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241722B8

Cat no : 84644-1-RR

Synonyms

TAPA1, S5.7, CD81 molecule, CD81 antigen, 26 kDa cell surface protein TAPA-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD81 Recombinant antibody (84644-1-RR)

    WB analysis using 84644-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84644-1-RR (CD81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84644-1-RR

    Affinity and Kinetic Characterization of 84644-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84644-1-RR against Human CD81 were performed. The affinity constant is 0.602 nM.

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Tested Applications

Positive WB detected inRaji cells, Ramos cells, Daudi cells, Jurkat cells, THP-1 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84644-1-RR is CD81 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD81 molecule
Calculated molecular weight26kDa
Observed molecular weight20 kDa
GenBank accession numberNM_004356.4
Gene symbol CD81
Gene ID (NCBI) 975
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Protocols

Product Specific Protocols
WB protocol for CD81 antibody 84644-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols