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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD82 Polyclonal antibody, PBS Only

CD82 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10248-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using CD82 Polyclonal antibody (10248-1-AP)

    WB analysis of K-562 using 10248-1-AP (same clone as 10248-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 10248-1-AP (CD82 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CD82 Polyclonal antibody (10248-1-AP)

    WB analysis of mouse brain using 10248-1-AP (same clone as 10248-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10248-1-AP (CD82 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD82 Polyclonal antibody (10248-1-AP)

    IHC staining of human tonsillitis using 10248-1-AP (same clone as 10248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD82 Polyclonal antibody (10248-1-AP)

    IHC staining of human tonsillitis using 10248-1-AP (same clone as 10248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CD82 Polyclonal antibody (10248-1-AP)

    IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CD82 Polyclonal antibody (10248-1-AP)

    IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD82 Polyclonal antibody (10248-1-AP)

    IHC staining of human breast cancer using 10248-1-AP (same clone as 10248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD82 Polyclonal antibody (10248-1-AP)

    IHC staining of human breast cancer using 10248-1-AP (same clone as 10248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CD82 Polyclonal antibody (10248-1-AP)

    IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using CD82 Polyclonal antibody (10248-1-AP)

    IHC staining of human thyroid cancer using 10248-1-AP (same clone as 10248-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10248-1-AP (CD82 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10248-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10248-1-PBS targets CD82 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD82 fusion protein Ag0314 相同性解析による交差性が予測される生物種
Full Name CD82 molecule
Calculated molecular weight 30 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC000726
Gene symbol CD82
Gene ID (NCBI) 3732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.