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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD83 Polyclonal antibody

CD83 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 32149-1-AP

Synonyms

Cell surface protein HB15, CD83 molecule, CD83 antigen, BL11, B-cell activation protein

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD83 Polyclonal antibody (32149-1-AP)

    IHC staining of human tonsillitis using 32149-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32149-1-AP (CD83 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD83 Polyclonal antibody (32149-1-AP)

    IHC staining of human tonsillitis using 32149-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32149-1-AP (CD83 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32149-1-AP targets CD83 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD83 molecule
Calculated molecular weight23 kDa
GenBank accession numberNM_004233.4
Gene symbol CD83
Gene ID (NCBI) 9308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).

Protocols

Product Specific Protocols
IHC protocol for CD83 antibody 32149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols