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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD84 Recombinant antibody, PBS Only (Detector)

CD84 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241342F3

Cat no : 84245-2-PBS

Synonyms

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD84 Recombinant antibody (84245-2-RR)

    WB analysis using 84245-2-RR (same clone as 84245-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84245-2-RR (CD84 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84245-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD84 Recombinant antibody (84245-2-RR)

    IF Staining of Raji using 84245-2-RR (same clone as 84245-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD84 antibody (84245-2-RR, Clone: 241342F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84245-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01112-1

    Cytometric bead array standard curve of MP01112-1

    Cytometric bead array standard curve of MP01112-1, CD84 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98108-1-PBS. Detection antibody: 84245-2-PBS. Standard: RP02347. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01112-3

    Cytometric bead array standard curve of MP01112-3

    Cytometric bead array standard curve of MP01112-3, CD84 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84245-3-PBS. Detection antibody: 84245-2-PBS. Standard: RP02347. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84245-2-RR

    Affinity and Kinetic Characterization of 84245-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84245-2-RR against Human CD84 were performed. The affinity constant is 0.409 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84245-2-PBS targets CD84 as part of a matched antibody pair:

MP01112-1: 98108-1-PBS capture and 84245-2-PBS detection (validated in Cytometric bead array)

MP01112-3: 84245-3-PBS capture and 84245-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD84 molecule
Calculated molecular weight 345 aa, 39 kDa
GenBank accession numberBC020063
Gene symbol CD84
Gene ID (NCBI) 8832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).