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Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01112-2

    Sandwich ELISA standard curve of MP01112-2

    Sandwich ELISA standard curve of MP01112-2, Human CD84 Recombinant Matched Antibody Pair - PBS only. 84245-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02347. 98108-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP01112-2

    Sample test of MP01112-2

    The mean CD84 concentration was determined to be 59.65 ng/mL in Raji cell extract based on a 1.0 mg/mL extract load and 79.95 ng/mL in Daudi cell extract based on a 1.8 mg/mL extract load.

  • Sample test of MP01112-2

    Sample test of MP01112-2

    Serum of six individual healthy human donors was measured. The human CD84 concentration of detected samples was determined to be 13,292.9 pg/mL with a range of 8,538.9 - 16,254.6 pg/mL.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR)

    FC experiment of human PBMCs using 98108-1-RR (same clone as 98108-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR, Clone: 241345A3) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD4 (OKT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98108-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR)

    FC experiment of human PBMCs using 98108-1-RR (same clone as 98108-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR, Clone: 241345A3) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98108-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01112-1

    Cytometric bead array standard curve of MP01112-1

    Cytometric bead array standard curve of MP01112-1, CD84 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98108-1-PBS. Detection antibody: 84245-2-PBS. Standard: RP02347. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01112-2

    Cytometric bead array standard curve of MP01112-2

    Cytometric bead array standard curve of MP01112-2, CD84 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84245-1-PBS. Detection antibody: 98108-1-PBS. Standard: RP02347. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 98108-1-RR

    Affinity and Kinetic Characterization of 98108-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98108-1-RR against Human CD84 were performed. The affinity constant is 1.14 nM.

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Anti-Human CD84 Rabbit Recombinant Antibody, PBS Only

CD84 Uni-rAbTM Recombinant Antibody for FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 98108-1-PBS
Clone No.241345A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98108-1-PBS targets CD84 as part of a matched antibody pair:

MP01112-1: 98108-1-PBS capture and 84245-2-PBS detection (validated in Cytometric bead array)

MP01112-2: 84245-1-PBS capture and 98108-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD84 molecule
Calculated molecular weight 345 aa, 39 kDa
GenBank accession numberBC020063
Gene Symbol CD84
Gene ID (NCBI) 8832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UIB8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).