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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody

CD85j / LILRB1 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/75

Cat no : 65623-1-MR

Synonyms

LILRB1, ILT 2, GHI/75, FLJ37515, CD85j

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Rec (65623-1-MR)

    FC experiment of human PBMCs using 65623-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody (65623-1-MR, Clone: GHI/75) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Rec (65623-1-MR)

    FC experiment of human PBMCs using 65623-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD85j / LILRB1 (GHI/75) Mouse IgG2a Recombinant Antibody (65623-1-MR, Clone: GHI/75) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65623-1-MR targets CD85j / LILRB1 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Human Hairy Cell Leukemia Spleen Cells 相同性解析による交差性が予測される生物種
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Calculated molecular weight 71 kDa
GenBank accession numberBC015731
Gene symbol LILRB1
Gene ID (NCBI) 10859
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.

Protocols

Product Specific Protocols
FC protocol for CD85j / LILRB1 antibody 65623-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols