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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD86 Polyclonal antibody, PBS Only

CD86 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30691-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD86 Polyclonal antibody (30691-1-AP)

    WB analysis using 30691-1-AP (same clone as 30691-1-PBS)

    DC2.4 cells were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30691-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD86 Polyclonal antibody (30691-1-AP)

    WB analysis using 30691-1-AP (same clone as 30691-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30691-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30691-1-PBS targets CD86 in WB, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD86 fusion protein Ag33514 相同性解析による交差性が予測される生物種
Full Name CD86 antigen
Calculated molecular weight35 kDa
Observed molecular weight60 kDa
GenBank accession numberNM_019388
Gene symbol Cd86
Gene ID (NCBI) 12524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)