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Anti-Human CD86 (BU63)

CD86 Monoclonal Antibody for IF/ICC, FC
Cat No. 65165-1-Ig
Clone No.BU63

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

IF/ICC, FC

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive IF/ICC detected inRaji cells
Positive FC detected inDaudi cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65165-1-Ig targets CD86 in IF/ICC, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen B-lymphoblastoid cell line ARH 77 相同性解析による交差性が予測される生物種
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
GenBank accession numberBC040261
Gene Symbol CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
RRIDAB_2881481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42081
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Protocols

Product Specific Protocols
IF protocol for CD86 antibody 65165-1-IgDownload protocol
FC protocol for CD86 antibody 65165-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Agric Food Chem

Novel Collagen Analogs with Multicopy Mucin-Type Sequences for Multifunctional Enhancement Properties Using SUMO Fusion Tags

Authors - Zilong Zhao
humanFC

Int Immunopharmacol

Isosilybin A exhibits anti-inflammatory properties in rosacea by inhibiting MAPK pathway and M1 macrophage polarization

Authors - Chen-Chen Wu
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