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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD86 (BU63) Rabbit Recombinant Antibody, PBS Only

CD86 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

BU63

Cat no : 65514-1-PBS

Synonyms

Activation B7 2 antigen, B7 2, B70, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD86 (BU63) (65514-1-PBS)

    FC experiment of human PBMCs using 65514-1-PBS

    1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD86 (BU63) (65514-1-PBS)

    FC experiment of human PBMCs using 65514-1-PBS

    1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD86 (BU63) (65514-1-PBS)

    FC experiment of human PBMCs using 65514-1-PBS

    1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) (left and red), or stained with 0.1 ug Anti-Human CD86 Mouse Monoclonal Antibody (Clone:BU63) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Monocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65514-1-PBS targets CD86 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen B-lymphoblastoid cell line ARH 77 相同性解析による交差性が予測される生物種
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
GenBank accession numberBC040261
Gene symbol CD86
Gene ID (NCBI) 942
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Protocols

Product Specific Protocols
FC protocol for CD86 antibody 65514-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols