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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus Anti-Human CD86 (BU63) Rabbit Recombinant Antibody

CD86 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

BU63

Cat no : FITC-65514

Synonyms

Activation B7 2 antigen, B7 2, B70

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD86 (BU63) Rabbit Recombina (FITC-65514)

    FC experiment of human PBMCs using FITC-65514

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD86 (BU63) Rabbit Recombina (FITC-65514)

    FC experiment of human PBMCs using FITC-65514

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD86 (BU63) Rabbit Recombina (FITC-65514)

    FC experiment of human PBMCs using FITC-65514

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD86 Rabbit Recombinant Antibody (FITC-65514, Clone:BU63) or 5 ul FITC Plus Anti-Human CD86 Mouse Monoclonal Antibody (FITC-65165, Clone:BU63). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65514 targets CD86 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen B-lymphoblastoid cell line ARH 77 相同性解析による交差性が予測される生物種
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
GenBank accession numberBC040261
Gene symbol CD86
Gene ID (NCBI) 942
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD86 antibody FITC-65514Download protocol
Standard Protocols
Click here to view our Standard Protocols