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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD8b Monoclonal antibody

CD8b Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B3B8

Cat no : 68432-1-Ig

Synonyms

CD8, 4B3B8, Ly3, Leu2, CD8B1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD8b Monoclonal antibody (68432-1-Ig)

    IHC staining of human breast cancer using 68432-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68432-1-Ig (CD8b antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD8b Monoclonal antibody (68432-1-Ig)

    IHC staining of human breast cancer using 68432-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68432-1-Ig (CD8b antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68432-1-Ig targets CD8b in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD8b fusion protein Ag16925 相同性解析による交差性が予測される生物種
Full Name CD8b molecule
Calculated molecular weight 243 aa, 27 kDa
GenBank accession numberBC100913
Gene symbol CD8B
Gene ID (NCBI) 926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.

Protocols

Product Specific Protocols
IHC protocol for CD8b antibody 68432-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols