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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD8B Recombinant antibody, PBS Only (Capture)

CD8B Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241327C1

Cat no : 84240-3-PBS

Synonyms

CD8, 241327C1, Ly3, Leu2, CD8B1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01133-1

    Sandwich ELISA standard curve of MP01133-1

    Sandwich ELISA standard curve of MP01133-1, Human CD8b Recombinant Matched Antibody Pair - PBS only. 84240-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02351. 84240-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01133-1

    Sample test of MP01133-1

    Human peripheral blood mononuclear cells (PBMCs), with HeLa and HEK-293 as negative controls, were cultured for 5 days. The mean concentration of CD8b was measured 99.4 pg/mL in the PBMC supernatant, undetectable in HeLa and HEK-293 supernatants.

  • Sample test of MP01133-1

    Sample test of MP01133-1

    Serum of six individual healthy human donors was measured. The CD8b concentration of detected samples was determined to be 204 pg/mL with a range of 73-480 pg/mL.

  • Cytometric bead array standard curve of MP01133-1

    Cytometric bead array standard curve of MP01133-1

    Cytometric bead array standard curve of MP01133-1, CD8B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84240-3-PBS. Detection antibody: 84240-1-PBS. Standard: RP02351. Range: 0.078-10 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84240-3-PBS targets CD8B as part of a matched antibody pair:

MP01133-1: 84240-3-PBS capture and 84240-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD8b molecule
Calculated molecular weight 243 aa, 27 kDa
GenBank accession numberBC100913
Gene symbol CD8B
Gene ID (NCBI) 926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.