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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Antibody, PBS Only

CD8a Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT8

Cat no : 65506-1-PBS

Synonyms

CD8, p32, OKT8, MAL, Leu2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Anti (65506-1-PBS)

    FC experiment of human PBMCs using 65506-1-PBS

    1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD8a Rabbit Recombinant Antibody (65506-1-PBS, Clone:OKT8) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Anti (65506-1-RR)

    FC experiment of human PBMCs using 65506-1-RR (same clone as 65506-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD8a Rabbit Recombinant Antibody (65506-1-RR, Clone:OKT8) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.1 ug Anti-Human CD8a Mouse Monoclonal Antibody (Clone:OKT8) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65506-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Anti (65506-1-RR)

    FC experiment of human PBMCs using 65506-1-RR (same clone as 65506-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD8a Rabbit Recombinant Antibody (65506-1-RR, Clone:OKT8) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65506-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Anti (65506-1-PBS)

    FC experiment of human PBMCs using 65506-1-PBS

    1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD8a Rabbit Recombinant Antibody (65506-1-PBS, Clone:OKT8) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Anti (65506-1-PBS)

    FC experiment of human PBMCs using 65506-1-PBS

    1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD8a Rabbit Recombinant Antibody (65506-1-PBS, Clone:OKT8) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.1 ug Anti-Human CD8a Mouse Monoclonal Antibody (Clone:OKT8) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Anti (65506-1-RR)

    FC experiment of human PBMCs using 65506-1-RR (same clone as 65506-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD8a Rabbit Recombinant Antibody (65506-1-RR, Clone:OKT8) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.1 ug Anti-Human CD8a Mouse Monoclonal Antibody (Clone:OKT8) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65506-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 65506-1-RR

    Affinity and Kinetic Characterization of 65506-1-RR

    Biolayer interferometry (BLl) kinetic assays of 65506-1-RR against Human CD8a were performed. The affinity constant is 1.51 nM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65506-1-PBS targets CD8a in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Human thymocytes 相同性解析による交差性が予測される生物種
Full Name CD8a molecule
Calculated molecular weight 235 aa, 26 kDa
GenBank accession numberBC025715
Gene symbol CD8A
Gene ID (NCBI) 925
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T-cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
FC protocol for CD8a antibody 65506-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols