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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD8b Rabbit Recombinant Antibody

CD8b Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241326A7

Cat no : 98103-1-RR

Synonyms

CD8, 241326A7, Ly3, Leu2, CD8B1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD8b Rabbit Recombinant Antibody (98103-1-RR)

    FC experiment of human PBMCs using 98103-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8b Rabbit Recombinant Antibody (98103-1-RR, Clone: 241326A7) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD8b Rabbit Recombinant Antibody (98103-1-RR)

    FC experiment of human PBMCs using 98103-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8b Rabbit Recombinant Antibody (98103-1-RR, Clone: 241326A7)(red), or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98103-1-RR

    Affinity and Kinetic Characterization of 98103-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98103-1-RR against Human CD8b were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98103-1-RR targets CD8b in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD8b molecule
Calculated molecular weight 243 aa, 27 kDa
GenBank accession numberBC100913
Gene symbol CD8B
Gene ID (NCBI) 926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD8b (T-cell surface glycoprotein CD8 beta chain) is an integral membrane glycoprotein that forms disulfide-linked heterodimers with CD8a (CD8 alpha chain). The CD8 alpha/beta heterodimer is the predominant CD8 complex expressed on the cell surface (PMID: 1534146; 2111591). CD8 is a transmembrane glycoprotein predominantly expressed on the surface of cytotoxic T cells and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells.

Protocols

Product Specific Protocols
FC protocol for CD8b antibody 98103-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols