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CD9 Polyclonal antibody

CD9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20597-1-AP

Synonyms

CD9 molecule, CD9 antigen, BTCC 1, BA2, 5H9 antigen


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Tested Applications

Positive WB detected inHepG2 cells, mouse placenta tissue, RAW 264.7 cells, HeLa cells, L02 cells, human placenta tissue, mouse liver tissue, rat liver tissue, THP-1 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20597-1-AP targets CD9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, monkey, chicken, goat, bat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD9 fusion protein Ag14546 相同性解析による交差性が予測される生物種
Full Name CD9 molecule
Calculated molecular weight 228 aa, 25 kDa
Observed molecular weight 23-30 kDa
GenBank accession numberBC011988
Gene symbol CD9
Gene ID (NCBI) 928
RRIDAB_2878706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).

Protocols

Product Specific Protocols
WB protocol for CD9 antibody 20597-1-APDownload protocol
IHC protocol for CD9 antibody 20597-1-APDownload protocol
IF protocol for CD9 antibody 20597-1-APDownload protocol
FC protocol for CD9 antibody 20597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
humanWB

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu
WB

Bioact Mater

Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis

Authors - Wei Zhang
humanWB

J Extracell Vesicles

Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease

Authors - Rong Yang
human,monkeyWB,IP

EMBO J

Extracellular vesicles from Zika virus-infected cells display viral E protein that binds ZIKV-neutralizing antibodies to prevent infection enhancement

Authors - Fanfan Zhao
humanWB

J Exp Clin Cancer Res

Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization

Authors - Yuanshan Yao