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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CD9 Monoclonal antibody (60232-1-Ig)

    WB analysis of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)

    WB result of CD9 antibody (60232-1-Ig, 1:2000) with si-Control and si-CD9 transfected HeLa cells. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD9 Monoclonal antibody (60232-1-Ig)

    WB analysis using 60232-1-Ig (same clone as 60232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60232-1-Ig (CD9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IF Staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IF Staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IF Staining of human lung cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CD9 Monoclonal antibody (60232-1-Ig)

    IF Staining of human lung cancer using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CD9 Monoclonal antibody (60232-1-Ig)

    IF Staining of MCF-7 using 60232-1-Ig (same clone as 60232-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CD9 Monoclonal antibody, PBS Only

CD9 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 60232-1-PBS
Clone No.4H7B9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60232-1-PBS targets CD9 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD9 fusion protein Ag14529 相同性解析による交差性が予測される生物種
Full Name CD9 molecule
Calculated molecular weight 228 aa, 25 kDa
Observed molecular weight 23-27 kDa
GenBank accession numberBC011988
Gene Symbol CD9
Gene ID (NCBI) 928
RRIDAB_11232215
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21926
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.