Featured Product
KD/KO Validated

CD9 Monoclonal antibody, PBS Only

Name: CD9 Monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 60232-1-PBS

CD9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H7B9

Review Product

Cat no : 60232-1-PBS

Synonyms

5H9, 5H9 antigen, BA2, BTCC 1, CD9, CD9 antigen, CD9 molecule, Cell and organelle markers, DRAP 27, Exosome marker, GIG2, Leukocyte antigen MIC3, MIC3, Motility related protein, MRP 1, P24, Tetraspanin 29, Tspan 29, TSPAN29

Validation Data Gallery

Filter:

AllWBIHCIF

WB analysis of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)
WB result of CD9 antibody (60232-1-Ig, 1:2000) with si-Control and si-CD9 transfected HeLa cells. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
WB analysis using 60232-1-Ig (same clone as 60232-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60232-1-Ig (CD9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
×
Various lysates were subjected to SDS PAGE followed by western blot with 60232-1-Ig (CD9 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60232-1-Ig (CD9 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IF Staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IF Staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IF Staining of human lung cancer using 60232-1-Ig (same clone as 60232-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IF Staining of human lung cancer using 60232-1-Ig (same clone as 60232-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.
IF Staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD9 antibody (60232-1-Ig, Clone: 4H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60232-1-PBS in a different storage buffer formulation.

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Cat No. 60232-1-PBS

Size/Concentration

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Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60232-1-PBS targets CD9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CD9 fusion protein Ag14529 相同性解析による交差性が予測される生物種
Full Name	CD9 molecule
Calculated molecular weight	228 aa, 25 kDa
Observed molecular weight	23-27 kDa
GenBank accession number	BC011988
Gene symbol	CD9
Gene ID (NCBI)	928
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD9 Monoclonal antibody, PBS Only

CD9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Validation Data Gallery

WB analysis of HeLa using 60232-1-Ig (same clone as 60232-1-PBS)

WB analysis using 60232-1-Ig (same clone as 60232-1-PBS)

IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

IHC staining of human ovary tumor using 60232-1-Ig (same clone as 60232-1-PBS)

IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)

IHC staining of human colon cancer using 60232-1-Ig (same clone as 60232-1-PBS)

IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

IHC staining of human breast cancer using 60232-1-Ig (same clone as 60232-1-PBS)

IHC staining of human tonsillitis using 60232-1-Ig (same clone as 60232-1-PBS)