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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD9 Recombinant antibody

CD9 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240830A8

Cat no : 84142-1-RR

Synonyms

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD9 Recombinant antibody (84142-1-RR)

    WB analysis using 84142-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84142-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD9 Recombinant antibody (84142-1-RR)

    IHC staining of human ovary cancer using 84142-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84142-1-RR (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD9 Recombinant antibody (84142-1-RR)

    IHC staining of human ovary cancer using 84142-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84142-1-RR (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CD9 Recombinant antibody (84142-1-RR)

    IF Staining of MCF-7 using 84142-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (84142-1-RR, Clone: 240830A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84142-1-RR

    Affinity and Kinetic Characterization of 84142-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84142-1-RR against Human CD9 were performed. The affinity constant is 6.21 nM.

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Tested Applications

Positive WB detected inA431 cells, L02 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84142-1-RR is CD9 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD9 molecule
Calculated molecular weight25 kDa
Observed molecular weight23 kDa
GenBank accession numberNM_001769.4
Gene symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, which may be due to the difference in glycosylations (PMID: 8701996).

Protocols

Product Specific Protocols
WB protocol for CD9 antibody 84142-1-RRDownload protocol
IHC protocol for CD9 antibody 84142-1-RRDownload protocol
IF protocol for CD9 antibody 84142-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols