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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD9 Recombinant antibody

CD9 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242432C2

Cat no : 84801-4-RR

Synonyms

TSPAN29, Tspan 29, P24, MIC3, CD9 antigen

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD9 Recombinant antibody (84801-4-RR)

    IF Staining of RAW 264.7 using 84801-4-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD9 antibody (84801-4-RR, Clone: 242432C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84801-4-RR targets CD9 in IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name CD9 antigen
Calculated molecular weight25 kDa
GenBank accession numberNM_007657.3
Gene symbol Cd9
Gene ID (NCBI) 12527
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD9, also known as Tspan-29, p24 or MIC3, is a member of the tetraspanin superfamily (PMID: 1879540). It is expressed on a large variety of hematopoietic and non-hematopoietic cells, such as stromal cells, megakaryocytes, platelets, B and T lymphocytes, dendritic cells, endothelial cells, mast cells, eosinophils, and basophils (PMID: 30356731). CD9 interacts with the integrin family and other membrane proteins, and is involved in cell adhesion, cell motility and tumor metastasis (PMID: 8478605; 21428940; 25805926). Expression of CD9 enhances membrane fusion between muscle cells and supports myotube maintenance (PMID:10459022). On oocytes, CD9 is hypothesized to play a role in the fertilization of mammals (PMID: 25536312).

Protocols

Product Specific Protocols
IF protocol for CD9 antibody 84801-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols