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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse CD90.1 Recombinant antibody, PBS Only

CD90.1 Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241542D7

Cat no : 84832-1-PBS

Synonyms

CD90, Thy1, Thy 1, 241542D7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD90.1 Recombinant antibody (84832-1-RR)

    WB analysis using 84832-1-RR (same clone as 84832-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84832-1-RR (CD90.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84832-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84832-1-RR

    Affinity and Kinetic Characterization of 84832-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84832-1-RR against Mouse CD90.1 were performed. The affinity constant is 17.0 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84832-1-PBS targets CD90.1 in WB, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name thymus cell antigen 1, theta
Observed molecular weight25 kDa
Gene symbol CD90
Gene ID (NCBI) 21838
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR and FVB (PMID: 5919593).