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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD90 Polyclonal antibody

CD90 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31949-1-AP

Synonyms

Thy-1 membrane glycoprotein, Thy-1 antigen, Thy-1, Thy1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD90/Thy1 Polyclonal antibody (31949-1-AP)

    WB analysis using 31949-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31949-1-AP (CD90/Thy1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CD90/Thy1 Polyclonal antibody (31949-1-AP)

    IF Staining of PC-12 using 31949-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CD90/Thy1 antibody (31949-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inPC-12 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31949-1-AP targets CD90 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name Thy-1 cell surface antigen
Calculated molecular weight18 kDa
Observed molecular weight25 kDa
GenBank accession numberNM_012673.2
Gene symbol Thy1
Gene ID (NCBI) 24832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD90, also known as Thy1, is a 25-35 kDa protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

Protocols

Product Specific Protocols
WB protocol for CD90 antibody 31949-1-APDownload protocol
IF protocol for CD90 antibody 31949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols