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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD97 Polyclonal antibody (13071-1-AP)

    WB analysis using 13071-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CD97 Polyclonal antibody (13071-1-AP)

    WB analysis of Jurkat using 13071-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CD97 Polyclonal antibody (13071-1-AP)

    WB analysis of K-562 using 13071-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD97 Polyclonal antibody (13071-1-AP)

    WB analysis of U-937 using 13071-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 13071-1-AP (CD97 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD97 Polyclonal antibody (13071-1-AP)

    IHC staining of human tonsillitis using 13071-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD97 Polyclonal antibody (13071-1-AP)

    IHC staining of human tonsillitis using 13071-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13071-1-AP (CD97 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD97 Polyclonal antibody (13071-1-AP)

    IHC staining of human lymphoma using 13071-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13071-1-AP (CD97 antibody) at dilution of 1:100 (under 10x lens).

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CD97 Polyclonal antibody

CD97 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13071-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CD97 molecule, CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, U-937 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13071-1-AP targets CD97 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD97 fusion protein Ag3745 相同性解析による交差性が予測される生物種
Full Name CD97 molecule
Calculated molecular weight 722 aa, 79 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC026690
Gene Symbol CD97
Gene ID (NCBI) 976
RRIDAB_10646477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48960
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

Protocols

Product Specific Protocols
WB protocol for CD97 antibody 13071-1-APDownload protocol
IHC protocol for CD97 antibody 13071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols