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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using CD97 Monoclonal antibody (66972-1-Ig)

    WB analysis of K-562 using 66972-1-Ig (same clone as 66972-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using CD97 Monoclonal antibody (66972-1-Ig)

    WB analysis of HL-60 using 66972-1-Ig (same clone as 66972-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DC2.4 cells using CD97 Monoclonal antibody (66972-1-Ig)

    WB analysis of DC2.4 using 66972-1-Ig (same clone as 66972-1-PBS)

    DC2.4 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CD97 Monoclonal antibody (66972-1-Ig)

    WB analysis of HepG2 using 66972-1-Ig (same clone as 66972-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66972-1-Ig (CD97 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD97 Monoclonal antibody (66972-1-Ig)

    IF Staining of Jurkat using 66972-1-Ig (same clone as 66972-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD97 antibody (66972-1-Ig, Clone: 3F10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66972-1-PBS in a different storage buffer formulation.

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CD97 Monoclonal antibody, PBS Only

CD97 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66972-1-PBS
Clone No.3F10C2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66972-1-PBS targets CD97 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD97 fusion protein Ag27991 相同性解析による交差性が予測される生物種
Full Name CD97 molecule
Calculated molecular weight 722 aa, 79 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC026690
Gene Symbol CD97
Gene ID (NCBI) 976
RRIDAB_2882293
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48960
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).