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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD97 Recombinant antibody, PBS Only (Detector)

CD97 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241334A12

Cat no : 84255-1-PBS

Synonyms

CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5, ADGRE5

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01158-4

    Sandwich ELISA standard curve of MP01158-4

    Sandwich ELISA standard curve of MP01158-4, Human CD97 Recombinant Matched Antibody Pair - PBS only. 84255-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02330. 84255-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01158-4

    Sample test of MP01158-4

    The mean CD97 concentration was determined to be 11.39 ng/mL in HepG2 cell extract based on a 2.30 mg/mL extract load and 16.28 ng/mL in K-562 cell extract based on a 2.40 mg/mL extract load.

  • Sample test of MP01158-4

    Sample test of MP01158-4

    Serum of six mice was measured. The CD97 concentration of detected samples was determined to be 18.14 ng/mL with a range of 15.16-23.41 ng/mL.

  • Cytometric bead array standard curve of MP01158-2

    Cytometric bead array standard curve of MP01158-2

    Cytometric bead array standard curve of MP01158-2, CD97 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84255-2-PBS. Detection antibody: 84255-1-PBS. Standard: RP02330. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84255-1-PBS targets CD97 as part of a matched antibody pair:

MP01158-2: 84255-2-PBS capture and 84255-1-PBS detection (validated in Cytometric bead array)

MP01158-4: 84255-7-PBS capture and 84255-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD97 molecule
Calculated molecular weight 722 aa, 79 kDa
GenBank accession numberBC026690
Gene symbol CD97
Gene ID (NCBI) 976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.