Validation Data Gallery
Tested Applications
| Positive WB detected in | U-937 cells, DU 145 cells, BGC-823 cells, SGC-7901 cells, HL-60 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84255-6-RR targets CD97 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant Protein 相同性解析による交差性が予測される生物種 |
| Full Name | CD97 molecule |
| Calculated molecular weight | 722 aa, 79 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | BC026690 |
| Gene Symbol | CD97 |
| Gene ID (NCBI) | 976 |
| RRID | AB_3671803 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P48960 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD97 antibody 84255-6-RR | Download protocol |
| IHC protocol for CD97 antibody 84255-6-RR | Download protocol |
| IF protocol for CD97 antibody 84255-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |