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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD97 Rabbit Recombinant Antibody

CD97 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241335D8

Cat no : 98094-2-RR

Synonyms

CD97 antigen, Adhesion G protein-coupled receptor E5 subunit beta, Adhesion G protein-coupled receptor E5 subunit alpha, Adhesion G protein-coupled receptor E5, ADGRE5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD97 Rabbit Recombinant Antibody (98094-2-RR)

    FC experiment of human PBMCs using 98094-2-RR

    1x10^6 human PBMCs were surface stained with 0.15 ug Anti-Human CD97 Rabbit Recombinant Antibody (98094-2-RR, Clone: 241335D8) or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD97 Rabbit Recombinant Antibody (98094-2-RR)

    FC experiment of human PBMCs using 98094-2-RR

    1x10^6 human PBMCs were surface stained with 0.15 ug Anti-Human CD97 Rabbit Recombinant Antibody (98094-2-RR, Clone: 241335D8) or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98094-2-RR

    Affinity and Kinetic Characterization of 98094-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98094-2-RR against Human CD97 were performed. The affinity constant is 27.6 nM.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98094-2-RR targets CD97 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD97 molecule
Calculated molecular weight 722 aa, 79 kDa
GenBank accession numberBC026690
Gene symbol CD97
Gene ID (NCBI) 976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).

Protocols

Product Specific Protocols
FC protocol for CD97 antibody 98094-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols