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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD98 Recombinant antibody

CD98 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241755A11

Cat no : 84387-6-RR

Synonyms

SLC3A2, MDU1, CD98/SLC3A2, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD98 Recombinant antibody (84387-6-RR)

    WB analysis using 84387-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84387-6-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CD98 Recombinant antibody (84387-6-RR)

    IF Staining of HepG2 using 84387-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD98 antibody (84387-6-RR, Clone: 241755A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84387-6-RR

    Affinity and Kinetic Characterization of 84387-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84387-6-RR against Human CD98 were performed. The affinity constant is 4.32 nM.

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Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84387-6-RR targets CD98 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated molecular weight68kDa
Observed molecular weight68-125 kDa
GenBank accession numberNM_002394.5
Gene symbol CD98
Gene ID (NCBI) 6520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD98 is a 125-kDa type II membrane glycoprotein heterodimer composed of a 40-kDa non-glycosylated light chain (amino-acid transporter) and an 85-kDa glycosylated heavy chain (CD98hc) (PMID: 11507094). CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). Molecular orientation studies have demonstrated that the C-terminal tail of human CD98 (hCD98), which contains a PDZ-binding domain, is extracellular (PMID: 18625289).

Protocols

Product Specific Protocols
WB protocol for CD98 antibody 84387-6-RRDownload protocol
IF protocol for CD98 antibody 84387-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols