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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse CD98/SLC3A2 Rabbit Recombinant Antibody

CD98/SLC3A2 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241247F1

Cat no : 98128-1-RR

Synonyms

Mdu1, CD98, Amino acid transporter heavy chain SLC3A2, 4F2hc, 4F2 cell-surface antigen heavy chain

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD98/SLC3A2 Rabbit Recombinant Antibody (98128-1-RR)

    FC experiment of mouse splenocytes using 98128-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD98/SLC3A2 Rabbit Recombinant Antibody (98128-1-RR, Clone: 241247F1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse TER-119 (TER-119) (APC-65149, Clone: TER-119). Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD98 Rabbit Recombinant Antibody (98128-1-RR)

    FC experiment of mouse splenocytes using 98128-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD98 Rabbit Recombinant Antibody (98128-1-RR, Clone: 241247F1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98128-1-RR

    Affinity and Kinetic Characterization of 98128-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98128-1-RR against Mouse CD98 were performed. The affinity constant is 7.51 nM.

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Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98128-1-RR is CD98/SLC3A2 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated molecular weight58 kDa
GenBank accession numberNM_008577.4
Gene symbol CD98/SLC3A2
Gene ID (NCBI) 17254
RRIDAB_3672274
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Protocols

Product Specific Protocols
FC protocol for CD98/SLC3A2 antibody 98128-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols