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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD98/SLC3A2 Monoclonal antibody (66883-1-Ig)

    WB analysis using 66883-1-Ig (same clone as 66883-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using CD98/SLC3A2 Monoclonal antibody (66883-1-Ig)

    IHC staining of human renal cell carcinoma using 66883-1-Ig (same clone as 66883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using CD98/SLC3A2 Monoclonal antibody (66883-1-Ig)

    IHC staining of human renal cell carcinoma using 66883-1-Ig (same clone as 66883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD98/SLC3A2 Monoclonal antibody (66883-1-Ig)

    IHC staining of human colon cancer using 66883-1-Ig (same clone as 66883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CD98/SLC3A2 Monoclonal antibody (66883-1-Ig)

    IHC staining of human cervical cancer using 66883-1-Ig (same clone as 66883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CD98/SLC3A2 Monoclonal antibody (66883-1-Ig)

    IHC staining of human cervical cancer using 66883-1-Ig (same clone as 66883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD98/SLC3A2 Monoclonal antibody (66883-1-Ig)

    IHC staining of human liver cancer using 66883-1-Ig (same clone as 66883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD98/SLC3A2 Monoclonal antibody (66883-1-Ig)

    IHC staining of human colon cancer using 66883-1-Ig (same clone as 66883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD98/SLC3A2 Monoclonal antibody (66883-1-Ig)

    IHC staining of human liver cancer using 66883-1-Ig (same clone as 66883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66883-1-Ig (CD98/SLC3A2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CD98/SLC3A2 Monoclonal antibody (66883-1-Ig)

    IF Staining of HeLa using 66883-1-Ig (same clone as 66883-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CD98/SLC3A2 antibody (66883-1-Ig, Clone: 2B10F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66883-1-PBS in a different storage buffer formulation.

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CD98/SLC3A2 Monoclonal antibody, PBS Only

CD98/SLC3A2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66883-1-PBS
Clone No.2B10F5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD98, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66883-1-PBS targets CD98/SLC3A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD98/SLC3A2 fusion protein Ag7301 相同性解析による交差性が予測される生物種
Full Name solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
Calculated molecular weight 68 kDa
Observed molecular weight 85-94 kDa, 120-130 kDa
GenBank accession numberBC001061
Gene Symbol CD98
Gene ID (NCBI) 6520
RRIDAB_2882215
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08195
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).