Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, U-937 cells, THP-1 cells |
| Positive IHC detected in | human pancreas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
23079-1-AP targets CD99 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD99 fusion protein Ag19461 相同性解析による交差性が予測される生物種 |
| Full Name | CD99 molecule |
| Calculated molecular weight | 19 kDa, 16 kDa, 17 kDa |
| Observed molecular weight | 28 kDa, 32 kDa |
| GenBank accession number | BC021620 |
| Gene Symbol | CD99 |
| Gene ID (NCBI) | 4267 |
| RRID | AB_2879205 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P14209 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD99 antibody 23079-1-AP | Download protocol |
| IHC protocol for CD99 antibody 23079-1-AP | Download protocol |
| FC protocol for CD99 antibody 23079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Anim Sci Biotechnol Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis. | ||
Oncol Lett Metastatic extraskeletal Ewing's sarcoma treated with trabectedin: A case report. | ||