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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD99 Monoclonal antibody, PBS Only

CD99 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B6A4

Cat no : 60354-1-PBS

Synonyms

12E7, CD99, CD99 antigen, CD99 molecule, E2 antigen, MIC2, MIC2X, MIC2Y, Protein MIC2, T cell surface glycoprotein E2

Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using CD99 Monoclonal antibody (60354-1-Ig)

    WB analysis of human spleen using 60354-1-Ig (same clone as 60354-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 60354-1-Ig (CD99 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60354-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CD99 Monoclonal antibody (60354-1-Ig)

    WB analysis of HeLa using 60354-1-Ig (same clone as 60354-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60354-1-Ig (CD99 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60354-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60354-1-PBS targets CD99 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD99 fusion protein Ag19474 相同性解析による交差性が予測される生物種
Full Name CD99 molecule
Calculated molecular weight 19 kDa, 16 kDa, 17 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC021620
Gene symbol CD99
Gene ID (NCBI) 4267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing’s sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917). CD99 displays two surface isoforms generated by alternative splicing: a long 32 kDa and a short 28 kDa form (PMID: 12368226).