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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD99 Recombinant antibody

CD99 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241737H1

Cat no : 84580-4-RR

Synonyms

MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD99 Recombinant antibody (84580-4-RR)

    WB analysis using 84580-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84580-4-RR (CD99 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD99 Recombinant antibody (84580-4-RR)

    IF Staining of Jurkat using 84580-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD99 antibody (84580-4-RR, Clone: 241737H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inTHP-1 cells, Jurkat cells, HUVEC cells, U-937 cells, U-251 cells, MOLT-4 cells, human testis tissue
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84580-4-RR targets CD99 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD99 molecule
Calculated molecular weight 19 kDa, 16 kDa, 17 kDa
Observed molecular weight28 kDa, 32 kDa
GenBank accession numberBC021620
Gene symbol CD99
Gene ID (NCBI) 4267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).

Protocols

Product Specific Protocols
WB protocol for CD99 antibody 84580-4-RRDownload protocol
IF protocol for CD99 antibody 84580-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols