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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD99 Rabbit Recombinant Antibody, PBS Only

CD99 Recombinant Antibody for FC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241737H1

Cat no : 98174-1-PBS

Synonyms

MIC2, E2 antigen, CD99 molecule, CD99 antigen, 241737H1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR)

    FC experiment of human PBMCs using 98174-1-RR (same clone as 98174-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR, Clone:241737H1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98174-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR)

    FC experiment of human PBMCs using 98174-1-RR (same clone as 98174-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD99 Rabbit Recombinant Antibody (98174-1-RR, Clone:241737H1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98174-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01403-1

    Cytometric bead array standard curve of MP01403-1

    Cytometric bead array standard curve of MP01403-1, CD99 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98174-1-PBS. Detection antibody: 84580-1-PBS. Standard: RP02321. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 98174-1-RR

    Affinity and Kinetic Characterization of 98174-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98174-1-RR against Human CD99 were performed. The affinity constant is 3.61 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98174-1-PBS targets CD99 as part of a matched antibody pair:

MP01403-1: 98174-1-PBS capture and 84580-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name CD99 molecule
Calculated molecular weight 19 kDa, 16 kDa, 17 kDa
GenBank accession numberBC021620
Gene symbol CD99
Gene ID (NCBI) 4267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD99, also known as MIC2, is a heavily O-glycosylated transmembrane protein involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 is broadly distributed on many cell types, with particularly strong expression on human cortical thymocytes, Ewing's sarcoma cells and peripheral primitive neuroectodermal tumors (PMID: 9794396; 16984917). In normal cells, CD99 has been functionally implicated in cell adhesion, migration, apoptosis, differentiation, activation, and proliferation of lymphocytes and monocyte extravasation and transport of several transmembrane proteins (PMID: 16984917).