Validation Data Gallery
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Tested Applications
| Positive WB detected in | HUVEC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13732-1-AP targets CD99L2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD99L2 fusion protein Ag3809 相同性解析による交差性が予測される生物種 |
| Full Name | CD99 molecule-like 2 |
| Calculated molecular weight | 262 aa, 28 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC025729 |
| Gene Symbol | CD99L2 |
| Gene ID (NCBI) | 83692 |
| RRID | AB_3669170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TCZ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD99L2 (CD99 molecule like 2), also known as CD99B and MIC2L1, is a type 1 transmembrane protein that is similar to CD99 (PMID: 12706889). It is expressed on most leukocytes and several other cell types including neuronal cells (PMID: 12706889). CD99L2 may function as a homophilic adhesion molecule as well as play a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane (PMID: 37199607). Its related pathways respond to elevated platelet cytosolic Ca2+ and cell surface interactions at the vascular wall.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD99L2 antibody 13732-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |