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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CDA1 Polyclonal antibody (12087-2-AP)

    WB analysis of HeLa using 12087-2-AP (same clone as 12087-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CDA1 Polyclonal antibody (12087-2-AP)

    WB analysis of HEK-293 using 12087-2-AP (same clone as 12087-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CDA1 Polyclonal antibody (12087-2-AP)

    WB analysis of MCF-7 using 12087-2-AP (same clone as 12087-2-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using CDA1 Polyclonal antibody (12087-2-AP)

    WB analysis of DU 145 using 12087-2-AP (same clone as 12087-2-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CDA1 Polyclonal antibody (12087-2-AP)

    IP experiment of HEK-293 using 12087-2-AP (same clone as 12087-2-PBS)

    IP result of anti-CDA1 (IP:12087-2-AP, 4ug; Detection:12087-2-AP 1:1000) with HEK-293 cells lysate 1000ug. This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDA1 Polyclonal antibody (12087-2-AP)

    IHC staining of human lung cancer using 12087-2-AP (same clone as 12087-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12087-2-AP (CDA1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDA1 Polyclonal antibody (12087-2-AP)

    IF Staining of HeLa using 12087-2-AP (same clone as 12087-2-PBS)

    Immunofluorescent analysis of Hela cells, using TSPYL2 antibody 12087-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12087-2-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CDA1 Polyclonal antibody, PBS Only

CDA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12087-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12087-2-PBS targets CDA1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDA1 fusion protein Ag2722 相同性解析による交差性が予測される生物種
Full Name TSPY-like 2
Calculated molecular weight 693 aa, 79 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC024270
Gene Symbol CDA1
Gene ID (NCBI) 64061
RRIDAB_2256811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2G4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TSPYL2 (also known as CINAP, CDA1, TSPX or DENTT) is a new member of the nucleosome assembly protein superfamily. TSPYL2 binds histones and facilitates nucleosome assembly. TSPYL2 is expressed in various tissues, highly in the pituitary gland and moderately in the adrenals, brain, testis, and ovary. Immunohistochemical staining analysis for TSPYL2 showed differential cytoplasmic and nuclear staining patterns in several cell types. Downregulated expression of TSPYL2 has been observed in several tumors, which suggests its role as a tumor suppressor. Although it is predicted that TSPYL2 has a molecular mass of 79.43 kDa, it is found that mammalian TSPYL2 appears at a size of 120 kDa by western blot analysis. The abundant acidic amino acid regions in TSPYL2 may cause its aberrant migration. In addition, the TSPYL2 protein is unstable and sensitive to proteasomal degradation.