• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDC16/APC6 Polyclonal antibody

CDC16/APC6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14307-1-AP

Synonyms

CDC16, APC6, CDC16Hs, CDC16 homolog, Anaphase-promoting complex subunit 6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    WB analysis using 14307-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    WB analysis of HeLa using 14307-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    WB analysis of K-562 using 14307-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    WB analysis of NIH/3T3 using 14307-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14307-1-AP (CDC16/APC6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    IHC staining of human cervical cancer using 14307-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    IHC staining of human cervical cancer using 14307-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14307-1-AP (CDC16/APC6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDC16/APC6 Polyclonal antibody (14307-1-AP)

    IF Staining of HeLa using 14307-1-AP

    Immunofluorescent analysis of Hela cells, using CDC16 antibody 14307-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells, Jurakt cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14307-1-AP targets CDC16/APC6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC16/APC6 fusion protein Ag5427 相同性解析による交差性が予測される生物種
Full Name cell division cycle 16 homolog (S. cerevisiae)
Calculated molecular weight 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC010875
Gene symbol CDC16/APC6
Gene ID (NCBI) 8881
RRIDAB_2074638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
IHC protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
IF protocol for CDC16/APC6 antibody 14307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols