Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10252-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10252-1-AP
WB result of Cdc20 antibody (10252-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cdc20 transfected HeLa cells.
WB analysis using 10252-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 10252-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10252-1-AP (Cdc20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 10252-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 10252-1-AP (CDC20 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10252-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10252-1-AP (CDC20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 10252-1-AP
IP result of anti-Cdc20 (IP:10252-1-AP, 4ug; Detection:10252-1-AP 1:15000) with Jurkat cells lysate 1400 ug.
IHC staining of human breast cancer using 10252-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10252-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10252-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (Cdc20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10252-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10252-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 10252-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 10252-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10252-1-AP (CDC20 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10252-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cdc20 antibody (10252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Cdc20 Polyclonal antibody

Cdc20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10252-1-AP

Publications(40)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

p55CDC, CDC20A

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (40)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, PC-3 cells, HL-60 cells, Jurkat cells, HepG2 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:14000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 33 publications below
IHC	See 11 publications below
IF	See 4 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10252-1-AP targets Cdc20 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cdc20 fusion protein Ag0295 相同性解析による交差性が予測される生物種
Full Name	cell division cycle 20 homolog (S. cerevisiae)
Calculated molecular weight	55 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC001088
Gene Symbol	CDC20
Gene ID (NCBI)	991
RRID	AB_2229016
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q12834
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

Protocols

Product Specific Protocols
WB protocol for Cdc20 antibody 10252-1-AP	Download protocol
IHC protocol for Cdc20 antibody 10252-1-AP	Download protocol
IF protocol for Cdc20 antibody 10252-1-AP	Download protocol
IP protocol for Cdc20 antibody 10252-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation Authors - Xiaohua Xu View Article
human	IF	Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. Authors - Hagai Marmor-Kollet View Article
human	WB	Sci Signal Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas Authors - Matthew J Knarr View Article
human	WB	Elife Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit. Authors - James Holder View Article
human	WB	Int J Mol Sci Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells. Authors - Yang Gao KD Validated View Article
human	WB	Cell Death Dis Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma. Authors - Yanlin Huang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10252-1-AP

WB analysis of HeLa using 10252-1-AP

WB analysis using 10252-1-AP

WB analysis using 10252-1-AP

WB analysis of HL-60 using 10252-1-AP

WB analysis of PC-3 using 10252-1-AP

IP experiment of Jurkat using 10252-1-AP

IHC staining of human breast cancer using 10252-1-AP

IHC staining of human breast cancer using 10252-1-AP

IHC staining of human colon cancer using 10252-1-AP

IHC staining of human colon cancer using 10252-1-AP

IHC staining of human colon cancer using 10252-1-AP

IHC staining of human urothelial carcinoma using 10252-1-AP

IHC staining of human urothelial carcinoma using 10252-1-AP

IF Staining of HeLa using 10252-1-AP

Cdc20 Polyclonal antibody

Cdc20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Repurposing colforsin daropate to treat MYC-driven high-grade serous ovarian carcinomas

Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit.

Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells.

Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma.