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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDC20 Polyclonal antibody

CDC20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30451-1-AP

Synonyms

bA276H19.3, CDC20, CDC20A, p55CDC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC20 Polyclonal antibody (30451-1-AP)

    WB analysis using 30451-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30451-1-AP (CDC20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDC20 Polyclonal antibody (30451-1-AP)

    IHC staining of human colon cancer using 30451-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30451-1-AP (CDC20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CDC20 Polyclonal antibody (30451-1-AP)

    IF Staining of U2OS using 30451-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CDC20 antibody (30451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CDC20 Polyclonal antibody (30451-1-AP)

    IF Staining of HEK-293 using 30451-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CDC20 antibody (30451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDC20 Polyclonal antibody (30451-1-AP)

    IF Staining of HeLa using 30451-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CDC20 antibody (30451-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30451-1-AP targets CDC20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC20 fusion protein Ag32712 相同性解析による交差性が予測される生物種
Full Name cell division cycle 20 homolog (S. cerevisiae)
Calculated molecular weight 55 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC001088
Gene symbol CDC20
Gene ID (NCBI) 991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cdc20 (Cell-division cycle protein 20 homologue) is also named as p55CDC and belongs to the WD repeat CDC20/Fizzy family. Cdc20 has important functions in chromosome segregation and mitotic exit. Cdc20 is the target of the spindle assembly checkpoint (SAC) and a key cofactor of the anaphase-promoting complex or cyclosome (APC/CCDC20) E3 ubiquitin ligase, thus regulating APC/C ubiquitin activity on specific substrates for their subsequent degradation by the proteasome (PMID: 28202332). The 55-kDa band corresponded to the size of Cdc20 (PMID: 22566641).

Protocols

Product Specific Protocols
WB protocol for CDC20 antibody 30451-1-APDownload protocol
IHC protocol for CDC20 antibody 30451-1-APDownload protocol
IF protocol for CDC20 antibody 30451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols