Validation Data Gallery
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Tested Applications
| Positive WB detected in | SK-BR-3 cells, HeLa cells, SW480 cells, MOLT-4 cells, Jurkat cells, HSC-T6 cells, 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68457-1-Ig targets CDC25A in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle 25 homolog A (S. pombe) |
| Calculated molecular weight | 59 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | NM_001789 |
| Gene Symbol | CDC25A |
| Gene ID (NCBI) | 993 |
| RRID | AB_3085169 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P30304 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC25A antibody 68457-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |