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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDC25A Recombinant antibody

CDC25A Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240813F3

Cat no : 84145-2-RR

Synonyms

M-phase inducer phosphatase 1, EC:3.1.3.48, CDC25A2, CDC25, 240813F3

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDC25A Recombinant antibody (84145-2-RR)

    IF Staining of HepG2 using 84145-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDC25A Recombinant antibody (84145-2-RR)

    IF Staining of HepG2 using 84145-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC25A antibody (84145-2-RR, Clone: 240813F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84145-2-RR targets CDC25A in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cell division cycle 25 homolog A (S. pombe)
Calculated molecular weight 59 kDa
GenBank accession numberNM_001789
Gene symbol CDC25A
Gene ID (NCBI) 993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A.

Protocols

Product Specific Protocols
IF protocol for CDC25A antibody 84145-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols