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Validation Data Gallery

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  • Western Blot (WB) analysis of Sp2/0 cells using CDC25B Monoclonal antibody (67145-1-Ig)

    WB analysis of Sp2/0 using 67145-1-Ig (same clone as 67145-1-PBS)

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CDC25B Monoclonal antibody (67145-1-Ig)

    WB analysis of Jurkat using 67145-1-Ig (same clone as 67145-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using CDC25B Monoclonal antibody (67145-1-Ig)

    WB analysis of U-937 using 67145-1-Ig (same clone as 67145-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 67145-1-Ig (CDC25B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CDC25B Monoclonal antibody (67145-1-Ig)

    IHC staining of human skin cancer using 67145-1-Ig (same clone as 67145-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67145-1-Ig (CDC25B antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CDC25B Monoclonal antibody (67145-1-Ig)

    IF Staining of Jurkat using 67145-1-Ig (same clone as 67145-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CDC25B antibody (67145-1-Ig, Clone: 1H3A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67145-1-PBS in a different storage buffer formulation.

CDC25B Monoclonal antibody, PBS Only

CDC25B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67145-1-PBS
Clone No.1H3A4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

M phase inducer phosphatase 2, EC:3.1.3.48, Cell division cycle 25 homolog B, CDC25HU2, Cdc 25B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67145-1-PBS targets CDC25B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27193

Product name: Recombinant human CDC25B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 169-398 aa of BC006395

Sequence: SQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSK

相同性解析による交差性が予測される生物種
Full Name cell division cycle 25 homolog B (S. pombe)
Calculated molecular weight 65 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC006395
Gene Symbol CDC25B
Gene ID (NCBI) 994
RRIDAB_2882444
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP30305
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

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